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Tag Archives: UCE
RADseq vs. UCEs, round 3
Though reduced-representation genome sequencing (or high-throughput, or nextgen, or massively parallel sequencing, or…) has become standard practice for molecular ecology labs over the past few years, the relative merits of different library preparation methods remains an active area of research. … Continue reading →
Posted in bioinformatics, evolution, genomics, methods, next generation sequencing, phylogenetics
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Tagged Piranga, RADseq, UCE, ultraconserved elements
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1 Comment
Comparing your options for phylogenomic data
The choices for current-generation (last generation?) molecular markers are grouped in two primary camps. First, the “reduced representation” methods: take some DNA, cut it up with specific enzymes, tag those pieces, read the sequences. These methods produce lots and lots of … Continue reading →
Posted in methods, next generation sequencing, phylogenetics
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Tagged phylogenomics, RADseq, UCE
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2 Comments
The Goldilocks zone of missing data
Reduced representation sequencing approaches, such as RADseq and UCEs, have provided some fascinating inferences in recent years, but something has always been missing in these analyses: data. As sampled taxa become more divergent, the price paid for more loci is … Continue reading →
Posted in evolution, methods, next generation sequencing, phylogenetics
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Tagged lizards, RADseq, reduced-representation sequencing, UCE
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1 Comment