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Category Archives: RNAseq
Still ruffling feathers after all these years: Darwin's finches and a molecular view of adaptive radiation
One of the many lovely things about molecular ecology is its ability to shine new light on old stories. The well-known case of Darwin’s finches is a classic example of adaptive radiation. These finches demonstrate a clear instance where over time, … Continue reading
Respect the old but seek out the new: Direct 16S rRNA-seq from bacterial communities
I think it’s fair to say that it’s an ongoing struggle to figure out what the heck microbes are doing in their natural environments, and who those microbes are. Clearly, there is no silver bullet that gives us all the … Continue reading
Posted in community ecology, microbiology, RNAseq
Tagged 16S rRNA, microbial community analysis, pyrosequencing, RNA-seq
1 Comment
The 2016 Next-Generation Sequencing Field Guide Preview: Zombie Systems and New Hope
After a year of minimal activity, we finally have some significant changes in Next Gen Land. In the 2016 update of the NGS Field Guide, I will continue to give my overall interpretation about the various instruments, but with less … Continue reading
Posted in genomics, howto, methods, RNAseq, transcriptomics
Tagged Illumina, Ion Torrent, NGS Field Guide, PacBio
8 Comments
Understanding amphibian disease inside out
In the spring of 2010, I was doing amphibian surveys among a few wetlands in Eastern Kentucky that were known for their excellent diversity. As I sauntered up to a familiar study site, I was greeted with an amphibian massacre. Hundreds of … Continue reading