Category Archives: methods

Data archiving guide

Posted on 24 Jun, 2011 by Tim Vines

I put together a short guide that goes through the different data types commonly used in Molecular Ecology and suggests the type of file that ought to be archived to fulfil our Joint Data Archiving Policy. It’s available here. Comments … Continue reading →

Posted in methods, science publishing | Leave a comment

Field Guide to Next Generation Sequencers now available

Posted on 8 Jun, 2011 by Tim Vines

Travis Glenn recently published a paper in ME Resources giving pricing and usage info for a range of NGS sequencers. Since this area is moving very fast, we’ve also put the tables from his paper up on the blog, and … Continue reading →

Posted in bioinformatics, howto, methods, next generation sequencing | 2 Comments

A penny for your method: Plate sealers

Posted on 8 Apr, 2011 by Brant Faircloth

You may call them plate sealers, we call them “sticky-tops” (as opposed to the rubber plate sealers we use for PCR). Whatever you call them, they seal up 96- and 384-well plates so you can store them in the fridge … Continue reading →

Posted in howto, methods | 1 Comment

Get your protein

Posted on 3 Apr, 2011 by Brant Faircloth

This weekend, I was doing a little work on one of our projects where we are using various cpDNA genes. I really needed to get a number of protein sequences from Genbank for the products of chloroplast genes. If I … Continue reading →

Posted in bioinformatics, howto, methods | 2 Comments

A new poll for our readers.

Posted on 31 Mar, 2011 by Dilara Ally

We all know that the genetic landscape has dramatically changed in the last five years with the introduction of multiple parallel sequencing platforms. What I’m interested in is which platform researchers are choosing to use. You might say that in … Continue reading →

Posted in community, methods, next generation sequencing | 2 Comments

Should I use FST, G'ST or D?

Posted on 2 Mar, 2011 by Nolan Kane

I have heard many researchers extolling one estimator over another, saying papers using any other approach should be rejected without review. Yet, although numerous recent papers assess various population genetic parameters and their validity in analyzing population structure, gene flow, … Continue reading →

Posted in methods, population genetics | Tagged , , | 23 Comments

A penny for your method: PCR cleanup

Posted on 19 Feb, 2011 by Brant Faircloth

Very rarely, in scientific research, do we get the opportunity to do less with more. As a result, one thing that we are good at is stretching a dollar. To (hopefully) contribute something back to the struggle, I’ve decided to … Continue reading →

Posted in methods | Tagged | 12 Comments